Apoptin induces apoptosis in human bladder cancer EJ and BIU-87 cells.
نویسندگان
چکیده
OBJECTIVE To investigate whether apoptin is a apoptosis-inducing protein with a potential for bladder cancer therapy. METHODS We constructed a PCDNA3/Apoptin eukaryotic expression vector, and transfected this vector into bladder cancer cell lines BIU-87 and EJ, then observed the results by RT-PCR, transmission electron microscopy, MTT assay and the flow cytometry (TUNEL method). RESULTS PCDNA3/Apoptin successfully induced a high level apoptosis in both bladder cancer cell lines, compared with the controls (p<0.05). CONCLUSIONS Apoptin can induce high level apoptosis in human bladder cancer EJ and BIU-87 cells, which suggests a potential for human bladder cancer therapy.
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عنوان ژورنال:
- Asian Pacific journal of cancer prevention : APJCP
دوره 13 1 شماره
صفحات -
تاریخ انتشار 2012